Identification, quantification and evaluation of angiogenic potential of carotenoids in malunggay leaves (Moringa oleifera Lamarck)

Date

10-2005

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Abstract

PREJILLANO, KAREN JOYCE A. College of Agriculture — College of Arts and Sciences. University of the Philippines Los Banos. October 2005. Identification, Quantification and Evaluation of Angiogenic Potential of Carotenoids in Malunggay Leaves (Moringa oleifera Lamarck).

ADVISER: Dr. Evelyn B. Rodriguez, Institute of Chemistry

Carotenoids belong to a group of phytochemicals known for their health promoting activities such as prevention of the risk of cancer and age-related macular degeneration. They are also recognized for their antioxidant properties, acting as quenchers of singlet oxygen and inhibitors of chain reactions leading to the formation of free radicals. Carotenoids are abundant in plant food like fruits and green leafy vegetables. In this study, carotenoids in malunggay leaves (Moringa oleifera Lamarck) were isolated, identified and quantified. The angiogenic potential of the carotenoids was also evaluated.

Carotenoids were extracted with cold acetone, partitioned to petroleum ether, concentrated in a rotary evaporator, and isolated by open column chromatography using MgO:celite (1:2) as adsorbent. The carotenoid fractions were collected and quantified by visible spectrophotometry. Chemical tests, their behavior in TLC and OCC, and their visible spectrum were the bases for identifying the individual carotenoids.

Four major carotenoids were obtained from malunggay leaves in the following elution sequence: 3-carotene, lutein, violaxanthin, and neoxanthin. Lutein was found to be the most predominant (61.64 ± 0.64 pg/g fresh sample) carotenoid, followed by (3-carotene (48.05 ± 2.03 gWg) which was the only carotene identified in the sample. Violaxanthin was also present (21.76 ± 0.74 gg/g) while neoxanthin has the lowest concentration (15.60 ± 1.00 ug/g).

The angiogenic potential of the carotenoids was evaluated following the duck embryo assay. Ba/ut samples were injected with the carotenoid extracts at 400, 800 and 1200 ppm concentration. The formation and suppression of blood vessels were observed after five days of incubation. Quercetin was used as standard. Results indicated that 3-carotene possessed proangiogenic effect while lutein exhibited inhibitory effect on angiogenesis. Eggs treated with the mixed carotenoid extract resulted in the death of the duck embryos.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2005 A13 P74

Document Type

Thesis

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