The use of lahar-silica gel mixtures as adsorbent in adsorption chromatography

Date

5-1996

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evamarie P. Capareda

Abstract

QUIATCHON JOSE ALBERTO V. University of the Philippines at Los Banos. May 1996. The Use of Lahar-Silica Gel Mixtures as Adsorbent In Adsorption Chromatography

Adviser Dr. Evamarie P. Capareda

The possible use of lahar as a chromatography adsorbent seas evaluated by comparing its performance with that of silica gel in both flash chromatography and TLC. When used as adsorbent in flash column chromatography of the 2.4-DNP derivative of benzaldehyde. lahar that was treated sequentially with H2S0, at 90 °C for 5 hrs. then aqua regia at 90 °C for 5 hrs. exhibited lower retention of the sample compared to silica gel. Similar results were obtained upon adding 11,02 to 1-1,S0., in the pre-treatment step.

A 50:50 mixture of the acid-treated lahar and silica gel 60 by weight gave an elution profile of the 2,4-DNP benzaldehyde derivative comparable to that of pure silica gel. A layered column of adsorbent consisting of silica gel on top of treated lahar (50:50 by height) performed just as well.

A mixture of three dyes - methyl red, bromcresol green, and phenol red, was separated by TLC using fine lahar particles as adsorbent and l -butanol:NR,OH:CHC13 (20:5:2) as the eluting solvent. However, Rf values were greater than those obtained on silica gel 60 G. Adjustment of the eluting solvent (to 15:3:2 ; 1-butanol:NR,OH:CHC13) resulted in better resolution and lower Rf's were obtained.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1996 C4 Q53

Document Type

Thesis

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