Isolation and optimization op lipase from Rhizopus oligosporus

Author

Belen B. Quintana

Date

6-1992

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Abstract

QUINTANA, BELEN BRILLO, College of Arts and Sciences, University of the Philippines at Los Banos, June 1992. Isolation and Optimization of Lipase From Rhizopus oligosporus.

ADVISER: DR. EUGENIA M. CASTILLO

The optimum experimental conditions for the production of lipase from Rhizopus oligosporus were determined. The organism was cultivated in a citrate-phosphate buffer - coconut oil medium for 8 days with continuous shaking at room temperature.

The lipase activity of Rhizopus oligosporus was determined in a cell-free broth of organism containing the enzyme. Optimum activity was obtained at pH 6.0, 35.C, for an incubation time of 40 minutes and its substrate-buffer ratio is 4:6.The hydrolyzed free fatty acids of coconut oil that were by Rhizopus oligosporus lipase were esterified and methyl esters formed were determined using gas chromatography.

The free fatty acids of coconut oil that were hydrolized by Rhizopus oligosporuslipase were esterified and methyl esters formed were determined using gas chromatography. The free fatty acids detected were caprylic (C₈), capric (C₁₀), lauric (C₁₂), myristic (C14), and palmitic (C₁₆).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1992 C4 Q56

Recommended Citation

Quintana, Belen B., "Isolation and optimization op lipase from Rhizopus oligosporus" (1992). Undergraduate Theses. 12865.
https://www.ukdr.uplb.edu.ph/etd-undergrad/12865

Document Type

Thesis