Studies on chloroform extract from tissue culture of Pittosporum resineferum Hemsl

Date

10-1988

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

William G. Padolina

Abstract

QUIZON, MARIA HELCARDA F., College of Arts and Sciences, University of the Philippines at Los Banos, October, 1988. Studies on the Chloroform Extract from Callus Culture of Pittosporum resineferum Hemsl.

Adviser: Dr. William G. Padolina

Light grown and dark grown callus of Pittosporum resineferum (Petroleum nut) cultured for 25, 30, 40, and 60 days in Lensmaier and Skoog culture medium were extracted with chloroform. Callus cultured for 30 days gave higher dry mass but the highest metabolite production was obtained from the 40-day cultures. However, no conclusion can be derived from the effect of light intensity on the yield of the extracts.

The constituents of the extract from 40 days callus culture were further isolated by thin layer chromatography.

Spectral data gave valuable information about the functional groups present in Fraction I and fraction 3 but did not allow full structural elucidation. The UV and IR spectra indicated the presence of ether functionalities and presence of methyl and methylene groups. The first fraction with Rf value of 0.05 showed presence of aromatic group while fraction 3 with Rf value of 0.52 indicated presence of olefinic group. UV-vis, IR, PER and mass spectral analysis of fraction 2 with Rf of 0.16 allowed full structural elucidation. The compound is proposed to be a phthalate ester (bis- 2 ethylhexyl phthalate). The occurrence of this as plant constituent is quite unexpected.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1988 A13 Q59

Document Type

Thesis

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