Semi-continuous fermentation of ethanol into acetic acid by immobilized Acetobacter

Date

5-1987

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J Del Rosario

Abstract

REAL, MA. PIA N. University of the Philippines at Los Banos. 1987. Semi-continuous Fermentation of Ethanol by Immobilized Acetobacter. Adviser: Dr. Ernesto J. del Rosario

A mixture of ethanol and coconut water was fermented into acetic acid using Acetobacter cells immobilized by adsorption on a solid support. Coconut husk and shell charcoals and vermi-culite were evaluated as immobilizing materials. The effects of nutrient addition to the medium and of variations in particle size and initial ethanol concentrations on the acetic acid pro-duction were also studied. Results showed that there was no significant difference in the acetic acid yield with or without nutrient addition to the ethanol substrate. The nutrients added were (in g/1): K2HPO4, 7H2O KH2PO4 , 0.9; (NH4) S2SO4 , 1.5; MgSO4 • 7H2O , 0.25; NaC1, 0.01; FeSO4,1.7H20, 0.01; MgSO4 20, 0.01; and yeast extract, 1.0. Similarly the results of acetic acid production were not statistically different using coconut husk charcoal, coconut shell charcoal or vermiculite as immobilizing material. How-ever, coconut husk charcoal as support gave a slightly higher acetic acid yield and was used for further optimization studies. The acetic acid productivity using smaller particles of coconut husk charcoal in a particle volume of 1 cm3, was not significantly different from that using bigger charcoal particles (4.5 cm3). Using small charcoal particles and 4% initial ethanol con-centration, total acidity )T/\( was 3.28 ± 0.03% with an a certification value (AV) of 66.01±0.50% after two days of culture, TA = 3.96 ± 0.02% and AV = 02.89 ± 0.62% after 8 days, and TA - 4.10 ± 0.02%, AV - 82.11± 0.75% after 10 clays, fermentation efficiencies were 50.78 ± 0.38%, 63.70 ± 0.48% and 72,06 Z ± 0,62% after two, eight and ten days of culture, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1987 C4 R43

Document Type

Thesis

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