"Separation of growth hormones in coconut water using micellar -enhanced ultrafiltration (with cetyl pyridinium chloride) through polyamide membranes"

Date

4-2004

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J Del Rosario

Abstract

Rivera, Kristine Joy Magracia. College of Arts and Sciences. University of the Philippines Los Banos. March 2004. Separation of Growth Hormones in Coconut Water using Micellar-Enhanced Ultrafiltration with Cetyl P Pyrdinium Chloride through Polvamide Membranes.

Adviser: Dr. Ernesto J. del Rosario

Two sets of polyamide ultrafiltration (UP) membranes were prepared using nylon 6 (polycaprolactam) as polymer substrate with water and ethanol as additives, and HC1- as depolymerizing solvent. The pinhole-free membranes were characterized in terms of thickness, water flux, membrane fouling, and molecular weight cut-off.

Membranes 1 and 2 had thickness of 0.18+0.03 and 0.17+0.04 mm, respectively. The average fluxes were 0.24+0.03 and 0.24+0.04 mUs for membranes 1 and 2, respectively. The percent rejection coefficients were 44% and 42% for membranes 1 and 2, respectively using bovine serum albumin (66.7 kD) as molecular weight (MW) standard and 53% and 51% using u-amylase (48.4 kD). The MW cut-off of the membranes was found to be 150 kD.

The prepared membranes were used to concentrate the growth hormones of coconut water in the retentate through micellar-enhanced ultrafiltration (MEUF) upon addition of cetyl pyridinium chloride (CPC). A tangential flow 1.1F set-up was used. A maximal value of 86% was obtained for the rejection coefficient at the critical micelle concentration (cmc) wherein the micelles trapped the growth hormones which were retained by the membrane. The rejection coefficients below the cmc were 79% and 80% for membranes 1 and 2, respectively. The rejection coefficient decreased at a CPC concentration above the cmc to 77% and 76% for membranes I and 2, respectively. This was due to the compression and disruption of the micelles which resulted in the release of growth hormones in the permeate.

Thin layer chromatography was used to separate the growth hormones. Kinetin and indoleacetic acid (IAA) were separated; the maximum recovery of kinetin and IAA in the retentate were 71.6% and 53.5%, respectively, which were obtained at the cmc of CPC.

Bioassay was done in order to test the effect of growth hormones in the hypocotyl elongation of Vigna radiata (mongo seeds). Based on the t-test for independent variables, there were significant differences at 95% confidence level between the control and the feed, as well as between permeate and retentate of the CPC solutions dissolved in coconut water.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS