Stability of plurilamellar liposomes containing bromophenol blue and pyrazinamide

Date

10-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

James A. Villanueva

Abstract

ABSTRACT

As an essential part of drug delivery systems. the encapsulation of all kinds of solutes into liposomes under every conceivable condition has become viable not only for research purposes but for economical and medical purposes as well. One of the biggest hurdles faced by liposomes is the maintenance of its stability in vivo, which is an interplay of a number of factors such as lipid fluidity, presence or absence of cholesterol, antioxidants, cryoprotectants, the prevention of lipid hydrolysis.

The focus of this paper is to study the effects of temperature and varying molar ratios of cholesterol and tocopherol succinate on the integrity of the lipid bilayer. Reverse evaporation method was used to encapsulate the solutes - bromophenol blue and pyrazinamide — using a 2:1 chloroform-methanol as solvent. The encapsulated materials in the samples were quantified using UV-Vis Spectroscopy and limiter characterized using differential scanning calorimetry.

For the bromophenol blue experiments, the highest percentage of encapsulation achieved was 61.88%. with the DPPC cholesterol tocopherol succinate ratio of 3:2:2 and the obtained transition temperature was 94.99°C. The AH obtained further validated the stability of this formulation as this was the same solution which had the highest AH at 428.5 J/g. In the case of the pyrazinamide experiments, the highest encapsulation percentage achieved was only 13.77%, with a ratio of 3:3:3 and an accompanying transition temperature of 98.58°C. The AH for this solution was not the highest obtained but was actually the lowest at 7.54 J/g.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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