Polyphenols in the leaves of mango (Mangifera indica, L.) var carabao, pico, indian and paho

Date

4-1999

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

RODIS, MARIDEL CUADRA. College of Arts and Sciences, University of the Philippines Los Banos, March 1999. POLYPHENOLS IN THE LEAVES OF MANGO (Mangifera, indica L.) var CARABAO, PICO, INDIAN AND PAHO.

Adviser: Dr. Evelyn Mae T. Mendoza

The contents of polyphenols in the leaves of different varieties of mango were determined by total phenol analysis, acidified vanillin and protein precipitation. The polyphenols were determined qualitatively by thin layer chromatography.

In the different varieties of mango leaves, the levels of total phenols in intermediate leaves were: 103.85 mg catechin/ g dry sample or catechin equivalence (CE) for Paho variety; 64.29 CE for Indian variety; 80.43 CE for Pico variety and 106.22 CE for Carabao variety. The flavan-type phenolics were 24.94 CE for Paho variety; 18.94 CE for Indian; 18.02 CE for Pico and 48.94 CE for Carabao. For protein precipitating polyphenols, the values were 13.09 mg tannic acid/ g dry sample (TAE) for Patio variety; 13.36 TAE for Indian; 13.76 TAE for Pico and 19.96 TAE for Carabao variety. Between Carabao variety and the three other varieties, the values were significantly different at 5% level.

In TLC (silica gel with 9:1 CHC13-CH3C001-1 as eluent), the Patio and Pico varieties both have 16 bands while Indian and Carabao both have 15 bands. Based from the standards available the probable identities of the bands were taxifolin, umbelliferone, gentisic acid, syringic acid and p-coumaric acid.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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