Immobilization of a lipase from Rhizopus delemar using celite

Date

3-1998

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Abstract

Romero, Marc Lawrence Jamisola. University of the Philippines Los Banos. March, 1998. Immobilization of a Lipase from Rhizopus delemar Using Celite.

Adviser: Eugenia M. Castillo, Ph. D.

Lipase from Rhizopus delemar was immobilized using Celite as solid support. Highest amount of enzyme adsorbed per gram of support was observed after 12 hours of incubation. Immobilization did not shift the optimum pH (5.0) and optimum temperature (30°C) of the free lipase. Storage stability of the immobilized lipase was observed and a gradual decrease of 25% in activity was noted as the days of storage were increased to 21 days. The immobilized lipase was subjected to five successive lipolytic activity assays and a drop in activity was observed after the fourth cycle with 26.4% retained relative activity.

Free and immobilized lipase were used to catalyze enzymatic glycerolysis of coconut oil in a solvent-free system. Thin layer chromatography and high pressure liquid chromatography were used to identify and quantify the products formed. . Results showed that higher yield of monoglyceride (1.2%) were obtained using immobilized lipase as catalyst.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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