"Separation of growth hormones in coconut water using micellar -enhanced ultrafiltration (with sodium dodecyl sulfate) through polyamide membranes"

Date

4-2004

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J Del Rosario

Abstract

SALISI, MARI SHERLIN DIMAANO, College of Arts and Sciences, University of the Philippines at Los Banos, April, 2004. Separation of Growth Hormones in Coconut Water Using Micellar-Enhanced Ultrafiltration (with Sodium Dodecyl Sulfate) through Polyamide Membranes.

Adviser: Dr. Ernesto J. del Rosario

Growth hormones, specifically indole-acetic acid and kinetin, were isolated from coconut water using micellar-enhanced ultrafiltration (MEUF) in the presence of sodium dodecyl sulfate (SDS), an anionic surfactant. Polymeric membranes were made from nylon 6 (polycaprolactam) pellets dissolved in an acid mixture. Upon preparation, the composition of the polymeric dope was 31.5% nylon 6, 2.5% ethanol, 25% water, 1% sulfuric acid, and 40% hydrochloric acid.

Membranes were characterized by water flux, fouling, pore diameter and molecular weight cut-off (MWCO). Characterizations for both membranes 1 and 2 were as follows: mean thickness of 0.20 mm; water flux of 0.076 . 0.0001 mL/s, and 0.0823 0.0001 mL/s, for membranes I and 2, respectively; solute rejection of 44.05 ± 0.85 and 49.25 ± 0.87 coefficient for Bovine Serum Albumin; solute rejection coefficient of 29.29 . 1.6 and 35.87 t 1.9 for a-amylase. Analysis of retentates and permeates from three concentrations of SDS-coconut water include bioassay with mongo seeds (Vigna radiata) and thin-layer chromatography (TLC). Low rejection values were obtained for SDS concentration below the critical micelle concentration (cmc) (86.92 . 0.14) since micelle formation is poor. However, rejection values are also low for concentration slightly higher than the cmc (98.16 ± 0.04). At this region, micelles are too large that they tend to squeeze out the solute particles. At the cmc, rejection coefficient was 99.44 ± 0.002.

TLC was used to quantify the growth hormones separated from coconut water. Percent recovery of these growth hormones were calculated for both permeate and retentate. Best results were obtained at the cmc of SDS which is 0.008 M: for indole-acetic acid, 57.6 . 0.1 (from permeate) and 42.4 ± 0.1 (from retentate); and for kinetin, 39.21 0.6 (from permeate) and 60.79 . 0.6 (from retentate).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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