Saccharification of corn cobs (Zea mays L.) using cellulase from Penicillium sp.

Date

11-1983

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Abstract

SAN LUIS, MARIA M. University of the Philippines at Los Banos, November 1983. Saccharification of Corn Cobs (Zea mays L.) Using Cellulase From Penicillium sp.

Adviser: Dr. Ernesto J. del Rosario

Trichoderma viride QM 9123, Aspergillus niger 330 and Penicillium sp. 86 were evaluated for cellulase production using corn cobs as carbon source. The three microorganisms showed maximum filter paper volumetric activity (FPVA) after 61 hours of shake flask culture. The highest activity obtained during the screening experiments was 0.119 IU/ml from Penicillium sp. 86. Production of cellulase by Penicillium sp. 86 in shake flask culture containing 2% ground corn cobs (40 mesh size) gave a FPVA of 0.14 IU/ml after 72 hours of batch culture.

A maximal saccharification value of 21.4% was obtained at pH. 4.8 and 50°C using 1% corn cobs. The saccharification value was increased by decreasing the amount of substrate and increasing the length of incubation. Ground corn cobs at 2.5% solids level showed 16% saccharification by Penicillium sp. 86 cellulase after incubation at 50oC, pH 4.8 for six hours. Alkali-treatment enhanced saccharification to 21.4 at 2.5% solids level and similar experimental conditions.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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