Voltammetric analysis of homocysteine in human blood plasma

Date

4-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Cherrie B. Pascual

Abstract

Voltammetric analysis of homocysteine was carried out in an electrochemical cell consisting of a platinum working electrode, Ag/AgCI reference electrode and a platinum wire auxiliary electrode. Both the cyclic and linear sweep voltammograms exhibited an anodic peak for homocysteine at around 800 mV. No characteristic cathodic peak was present. The anodic peak current was linearly proportional to (scan rate)'5 while the peak potential shifted to more positive values with increasing scan rate. These results indicate a simple irreversible oxidation reaction at the platinum working electrode. A linear and reproducible calibration curve response was obtained for homocysteine at its oxidation peak in the low concentration range of 1-10 ppm and high concentration range of 10-60 ppm homocysteine. The reproducibility test gave an average CV of 5.0% for the peak currents and an average CV of 1.5% for the anodic peak potential. Blood samples from healthy volunteers and volunteers with heart conditions were analyzed and gave homocysteine plasma concentrations ranging from 0.57 — 1.86 ug homocysteine / uL plasma, with an increase in the homocysteine level for the individuals with heart conditions.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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