Determination of copper from rice plant samples (Oryza sativa) using absorption spectroscopy

Date

4-2003

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Norriel S Nipales

Abstract

Saquilayan, Luanne Quiñones, College of Arts and Sciences, University of the Philippines Los Banos, April 2003. Determination of Copper from Rice Plant Samples (oryza sativa) using Absorption Spectroscopy.

Adviser: Dr. Norriel S. Nipales

The determination of the concentrations of copper in rice plants was conducted through the Absorption Spectroscopy, an extraction method using Sodium Diethyldithiocarbamate as complexing agent.

Rice plant samples were taken from a farm at Barangay Kilo-kilo in Sta. Cruz, a Municipality of Marinduque. The half-hectare rice field is situated adjacent to the main entrance of the Marinduque Mining Plant. The sampling was done at random from five different spots about ten (10) to twelve (12) meters apart.

Two methods were used in determining the Limit Of Detection (LOD) of each sample: 1.) The Visible Spectroscopy and 2.) The Flame Atomic Absorption Spectroscopy (FAAS). It was determined that the LOD values of the rice hulls and leaves using the Visible Spectroscopy method were notably lower than using the FAAS method. It was also noted that the detection limits of the rice grains and soil were higher when using the Visible Spectroscopic analysis while the results from the FAAS method were lower.

The copper concentrations obtained from both spectroscopic methods ranged from deficient to adequate levels. The copper concentrations of the rice grains and soil were within the adequate level whereas, those of the rice hulls and leaves were found to be in the deficient level. High percent recoveries of copper were obtained from all samples analyzed.

The statistical analysis, using the t-test method, showed that the values obtained using both Visible Spectroscopy and Flame Atomic Absorption Spectroscopy (FAAS) in the analysis of rice grains was not significantly different. On the other hand, the values in the analysis of rice hulls, leaves and soils were significantly different.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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