Isolation and characterization of Paenibacillus larvae from Philippine honeybees (Apis mellifera) and screening for their antibiotic resistance
Date
4-2010
Degree
Bachelor of Science in Biology
Major Course
Major in Microbiology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Noel G. Sabino
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Abstract
A total of 22 bacterial isolates were obtained from different apiaries in the Philippines. All the isolates produced creamy, convex, with entire margin colonies on YGSA. All were Gram positive rods which are capable of forming endospores. All the bacterial isolates were catalase negative, facultatively anaerobic, and incapable of degrading starch. However, only 12 are incapable of growing in Nutrient Broth and were screened for their resistance to three antibiotics commonly used by beekeeper namely: oxytetracycline, tylosin and erythromycin. It was found that all the 12 isolates were resistant to the antimicrobials. The isolate LU2 have exhibited the highest apparent resistance to oxytetracycline and erythromycin. Isolate BF1 showed the highest degree of resistance to tylosin while the rest were moderately resistant to the drug. The identity of the isolate LU2 was determined using the API 50 CHB kit. It was found that the fermentation pattern of the isolate is similar to that of Paenibacillus lautus, a soil and human intestinal tract inhabitant.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Recommended Citation
Consignado, Kryza I., "Isolation and characterization of Paenibacillus larvae from Philippine honeybees (Apis mellifera) and screening for their antibiotic resistance" (2010). Undergraduate Theses. 1300.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1300
Document Type
Thesis