Isolation and characterization of Paenibacillus larvae from Philippine honeybees (Apis mellifera) and screening for their antibiotic resistance

Date

4-2010

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Noel G. Sabino

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Abstract

A total of 22 bacterial isolates were obtained from different apiaries in the Philippines. All the isolates produced creamy, convex, with entire margin colonies on YGSA. All were Gram positive rods which are capable of forming endospores. All the bacterial isolates were catalase negative, facultatively anaerobic, and incapable of degrading starch. However, only 12 are incapable of growing in Nutrient Broth and were screened for their resistance to three antibiotics commonly used by beekeeper namely: oxytetracycline, tylosin and erythromycin. It was found that all the 12 isolates were resistant to the antimicrobials. The isolate LU2 have exhibited the highest apparent resistance to oxytetracycline and erythromycin. Isolate BF1 showed the highest degree of resistance to tylosin while the rest were moderately resistant to the drug. The identity of the isolate LU2 was determined using the API 50 CHB kit. It was found that the fermentation pattern of the isolate is similar to that of Paenibacillus lautus, a soil and human intestinal tract inhabitant.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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