Viscometric and spectroscopic (UV and IR) studies on the solvent free Maillard reaction (in melt) between xylose and glycine

Date

6-2001

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J Del Rosario

Abstract

SENICA, LAILANI P. University of the Philippines Los Banos. June 2001.Viscometrie and Spectroscopic (UV and IR) Studies on the Solvent Free Reaction (In Melt) Between Xylose and Glycine.

Adviser: Dr. Ernesto J. del Rosario

Committee members: Dr. Milagros M. Peralta Dr. Macario P. Catahan

Different mass ratios of xylose and glycine were thoroughly mixed in preweighed screw capped tubes. The change in mass of the mixture during heating at 155 °C was observed at different proportions of the reactants. The observed loss was found to be primarily due to the liberation of water.

The polymerization kinetics of the solvent-free reaction of xylose and glycine was studied viscometrically. Equimolar amounts of xylose and glycine were well mixed in clean screw capped tubes and heated in an insulated and well-stirred oil bath at 150, 155, 160 and 165°C. The effect of reaction time on the polymerization was determined at each temperature. Analysis revealed that the intrinsic viscosity of the samples heated at different time period increased with reaction time. This could be attributed to the polymerization of the samples as time progresses accompanied by an increase in the molecular weight (MW) of the product. The Mark Houwink constant (a), which is related on the molecular shape of the polymer, revealed that the Maillard reaction product was in random coil conformation at the different reaction temperatures studied.

The xylose-glycine Maillard reaction product at 155°C and 1 minute heating was purified using a Sephadex G-75 column buffered at pH 7 and then eluted from a DEAE-cellulose column with I M NaCI salt gradient. The molecular weight of the polymer product was found to be 36 700 Da.

The total nitrogen and carbon contents of the purified sample were determined and the C/N ratios were 14.42, 13.09, 21.68 and 28.33 for the products obtained at 150, 155, 160 and 165°C respectively.

The ultraviolet spectrum of the purified Maillard reaction product at 155°C was determined and showed absorption peaks at 208 and 196 mn. IR analysis of the samples showed the presence of C=C, -Ott and —CN groups.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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