Development of rapid method for the detection of ethylenebisdithiocarbamate fungicides

Date

4-1997

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Amelia W. Tejada

Abstract

SORIANO, NESTOR JR. URIAN. University of the Philippines at Los Banos, April, 1997. Development of Rapid Method for the Detection of Ethylenebisdithiocarbamate Fungicides. Thesis

Adviser: Dr. Amelia W. Tejada

A rapid method for the detection of ethylenebisdithiocarbamate (EBDC) fungicides was developed and the effects of acid concentration, duration of hydrolysis, and use of reducing agent on the quantitative evolution of carbon disulfide (CS2) during decomposition of EBDC at elevated temperature were determined. This method is based on the absorption of CS2 evolved with hexanes followed by the reaction of the CS2 absorbed with diethanolamine in the presence of cupric acetate to form a yellow complex suitable for colorimetric analysis.

Equilibrium condition for the quantitative evolution of CS2 was attained after 40 mins. of hydrolysis using 1% SnC12 in 4N Ha. The technique developed is applicable for detecting EBDC residues in fruits and vegetables.

Percent recovery developed ranged from 78.59% to 117.76%. The minimum detectable limit is about one microgram EBDC in one gram sample.

The rapid field kit that was packaged consist of the following: solution 1 (4N HC1), solution 2 (hexanes), solution 3 (color reagent), capsules containing SnC12, test tubes with screw caps, cutter, droppers, and forceps.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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