Development of a rapid method for the detection of carbamate and organophospate insecticides using enzyme- inhibition technique

Date

4-1995

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lilia S. P. Madamba

Co-adviser

Amelia W. Tejada

Abstract

TEJADA, FREDERICK RUBIO. University of the Philippines, April, 1995. Development of a rapid math.Q.ki for the detection at carbamate and organophosphate insecticides using enzyme inhibition technique.

Thesis advisers: Prof. Lilia S.P. Madamba Dr. Amelia W. Tejada

An enzymatic inhibition technique which is sensitive for the detection of carbamate and organophosphate insecticides was developed and packaged.

The sensitivity of the purified acetylcholinesterase (AChE) from Taiwan Agricultural Research Institute (TARI) housefly head was compared with that of the crude pig liver AChE. Housefly head AChE showed to be more sensitive than pig liver AChE which detected as low as 0.0016 ug and 0.0140 ug, respectively. Statistical analysis using Duncan Multiple Range Test showed that they are significantly different at 5% level.

TARI's housefly head AChE was used in determining the detectable amount of the organophosphate and carbamate insecticide residues in string bean samples ranging from 0.02 to 1.62 ug per gram of the string bean (Phaseolus sesguipedalis) sample.

The enzyme inhibition technique was packaged into a rapid test kit which consists of the following: substrate reagent capsules, powdered enzyme capsules, tris buffer, acetone, ethanol, enzyme solution container, substrate solution container , extraction vials and filter paper strips. Applicability of the method was used on spiked samples extracted with acetone. A white spot indicated the presence of the insecticide while a blue spot indicated its absence.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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