Extracellular lipase from Rhizopus sp. II. catalyzed preparation of methyl laurate

Date

5-1997

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Eugenia M. Castillo

Abstract

TERRADO, RHYAN JOSEPH SORIANO, College of Arts and Sciences, University of the Philippines at Los Banos, May, 1997. Extracellular Lipase From Rhizopus sp.11. Catalyzed Preparation of Methyl Laurate.

ADVISER: DR EUGENIA M. CASTILLO

Preliminary experiments involving the esterification of lauric acid with methanol catalyzed by extracellular lipase were carried out in closed flasks with shaking at room temperature. The effect of some parameters like the amount of water, methanol, enzyme, and incubation time were determined. The highest conversion yield for the effect of amount of methanol was 56.3 at 3.0 ml methanol and for the effect of amount of water, 52.9 % at 0.4 ml water. An increase in conversion yield was observed with an increase in the amount of enzyme used. Similar observations were noted for the effect of incubation time.

Thin layer chromatographic analysis of the product formed indicated the formation of methyl laurate. Further studies are needed to confirm the results.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS