An investigation of the possible allelopathic and molluscididal activity of Alpinia galanga Rhizomes

Date

10-2002

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn B. Rodriguez

Co-adviser

Evamarie P Capareda

Abstract

Crude extracts from langkauas ( Alpinia galanga ) rhizomes were obtained through sequential extraction with ethanol, ethyl acetate, and hexane. These crude extracts were evaluated for possible inhibitory effect on seed germination (allelopathic activity) and snail mortality (molluscicidal activity). The crude extracts were subjected to a seed germination assay, using radish ( Raphanus sativus L. ) seeds, and a snail contact toxicity assay, using the golden apple snail ( Pomacea canaliculata L.). The crude extracts showed almost no allelopathic activity based on the results of the seed aennination assay. The ethanol extract was found to exhibit 50 % snail mortality at 2000 ppm and 70% seed germination at 3000 ppm Chromatographic fractionation of the ethanol extract yielded two isolates, RTD and DIC. Isolate DIC was found to exhibit 50% snail mortality Isolate RTD was determined to have approximately 25% snail mortality. Spectroscopic analysis indicate that isolate DIC probably has an olefinic alcohol group while isolate RTD probably also has an olefinic alcohol with short-chain alkyl groups

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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