Evaluation of the analgesic potential of the skin secretions of ingerophynus philippinicus (Boulenger 1887)

Date

2010

Degree

Bachelor of Science in Biology

Major Course

Major in Zoology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ma. Dalisay G. Maligalig

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Abstract

The ether, ethyl acetate and aqueous fractions of the skin of Ingerophrynus philippinicus was evaluated for analgesic properties using the tail-flick method (fast pain) and abdominal writhing method (slow pain). Fifty mice were randomly distributed into an experimental set-up for each assay. Each set up includes five groups (n=5) namely: vehicle (corn oil), ether, ethyl acetate and aqueous fractions at 10ml kg-1 body weight; and a standard-morphine group (5.0 mg kg-1; oral and 0.8 mg kg-1; intraperitoneal, for tail flick and abdominal writhing, respectively). Results from the tail flick method showed no differences among the treatment groups for both mean reflex time and maximum possible analgesia (MPA). However, highest MPA was observed in the aqueous fraction. In the abdominal writhing method, the mean number of abdominal contractions in corn oil treated group (vehicle) was significantly different only from the animals in the standard –morphinie (p<0.05) but not in fraction groups. Comparison of % inhibition showed that values among the standard-morphine and the three fractions are closely similar. Of the three fractions, the aqueous fraction displayed a closer value with the standard group suggesting a similar effect to morphine. Thus, based on the MPA and % inhibition, there is a possible weak analgesic effect of the aqueous fraction of the skin secretion of I. philippinicus.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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