Modification of the rapid test kit for the detection of organophosphate and carbamate insecticides using enzyme inhibition technique

Date

3-1999

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Amelia W. Tejada

Abstract

VEJERANO, ERIC WARREN P., University ofthe Philippines Los Baños, March 1999. Modification of the Rapid Test Kit for the Detection of Organophosphate and Carbamate Insecticides Using Enzyme Inhibition Technique.

Thesis Adviser: Dr. Amelia W. Tejada

Modification of the rapid test for the detection of organophosphate and carbamate insecticides using an enzyme inhibition technique was done. This involved the substitution of crude housefly head acetylcholinesterase (AChE) as enzyme instead of Taiwan Agricultural Research Institute's (TARI) purified housefly head AChE.

Comparative study on the molar catalytic activities of crude housefly AChE and TARI's purified housefly AChE were determined to be 1.77 x 102 and 2.16 x 102 umole- ml¹-min¹, respectively. Activity loss of crude housefly AChE after one month and two months were found to be 30% and 52% of the original activity, respectively.

Values of the minimum detection limit (MDL) of purified housefly head AChE and crude housefly head AChE were determined using various inhibitors (organophosphate and carbamate insecticides). The MDL values for TARI's enzyme in increasing order were: carbaryl > carbofuran> chlorpyrifos > azinphos ethyl > methyl parathion > malathion > monocrotophos> methomyl> methamidophos. While the MDL values for the crude enzyme in increasing order were: carbaryl> carbofuran> chlorpyrifos >azinphos ethyl> methyl parathion, methomyl > malathion> methamidophos. Both enzymes detected as low as 0.0036 ug of pesticide. Analysis of Variance (ANOVA) showed that there was no significant difference between the enzymes used for carbaryl and chlorpyrifos at 5% level and methyl parathion and carbofuran at 1% level.

The results indicated that crude housefly extract can be used as a substitute enzyme for the rapid test kit for some specific pesticides only.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS