Isolation and identification of nitrogen-fixing bacteria from cavendish (Musa Acuminata (AAA Groups)) and evaluation of their effect on the growth of tissue-cultured banana plantlets under necessary conditions

Date

5-2014

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Nacita B. Lantican

Co-adviser

Juliet A. Anama

Abstract

Six nitrogen-fixing bacteria were isolated from the root samples of Cavendish bananas (Musa acuminata (AAA Groups)). Standard morphological protocols and biochemical characterization using automated VITEK® 2 Identification System were used to identify the isolates. The isolates were identified to be Elizabethkingia meningoseptica, Serratia marcescens, Neisseria zoodegmatis, Citrobacter freundii and Bacillus pumilus (two isolates, U2 and U5). The performance of the isolates was evaluated using tissue cultured Cavendish bananas as test plants under screenhouse conditions. Parameters gathered were plant height, diameter, and root and shoot fresh weight. Inoculation of Neisseria zoodegmatis was most effective in increasing the plants? height and diameter with 12.53% and 10.08% increases, respectively. The highest root and shoot dry weight were exhibited with plants treated with Serratia marcescens, with 64.29% and 10.26% increase, over the control, respectively. This is the first report on the possible nitrogen-fixing abilities of Neisseria zoodegmatis and Elizabethkingia meningoseptica. The results of the experiment revealed Neisseria zoodegmatis may have the potential to improve the growth of Cavendish bananas. Molecular studies and nitrogen-fixing evaluation methods should be conducted on the isolates to confirm the observations reported.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 B4 /D47

Document Type

Thesis

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