Isolation and identification of nitrogen-fixing bacteria from cavendish (Musa Acuminata (AAA Groups)) and evaluation of their effect on the growth of tissue-cultured banana plantlets under necessary conditions
Date
5-2014
Degree
Bachelor of Science in Biology
Major Course
Major in Microbiology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Nacita B. Lantican
Co-adviser
Juliet A. Anama
Abstract
Six nitrogen-fixing bacteria were isolated from the root samples of Cavendish bananas (Musa acuminata (AAA Groups)). Standard morphological protocols and biochemical characterization using automated VITEK® 2 Identification System were used to identify the isolates. The isolates were identified to be Elizabethkingia meningoseptica, Serratia marcescens, Neisseria zoodegmatis, Citrobacter freundii and Bacillus pumilus (two isolates, U2 and U5). The performance of the isolates was evaluated using tissue cultured Cavendish bananas as test plants under screenhouse conditions. Parameters gathered were plant height, diameter, and root and shoot fresh weight. Inoculation of Neisseria zoodegmatis was most effective in increasing the plants? height and diameter with 12.53% and 10.08% increases, respectively. The highest root and shoot dry weight were exhibited with plants treated with Serratia marcescens, with 64.29% and 10.26% increase, over the control, respectively. This is the first report on the possible nitrogen-fixing abilities of Neisseria zoodegmatis and Elizabethkingia meningoseptica. The results of the experiment revealed Neisseria zoodegmatis may have the potential to improve the growth of Cavendish bananas. Molecular studies and nitrogen-fixing evaluation methods should be conducted on the isolates to confirm the observations reported.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2014 B4 /D47
Recommended Citation
De Padua, Zared Patricia Patacsil, "Isolation and identification of nitrogen-fixing bacteria from cavendish (Musa Acuminata (AAA Groups)) and evaluation of their effect on the growth of tissue-cultured banana plantlets under necessary conditions" (2014). Undergraduate Theses. 1326.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1326
Document Type
Thesis