The isolation and characterization of chicken skin collagen and its use in the removal of inorganic arsenic from contaminated water

Date

10-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Milagros M. Peralta

Committee Member

Eden P. Sumalapao Josefina L. Solivas

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

CALVO, JENIFER SITON. College of Arts and Sciences. University of the Philippines Los Banos, October 2013. THE ISOLATION AND CHARACTERIZATION OF CHICKEN SKIN COLLAGEN AND ITS USE IN THE REMOVAL OF INORGANIC ARSENIC FROM CONTAMINATED WATER.

Adviser. MILAGROS M. PERALTA, PhD.

Collagen isolated from chicken skin was characterized, loaded with Fe(111) and tested for arsenic removal capacity using voltammetry. Collagen was isolated in 82.17% overall yield with a purity of 85.92% for the acid-extracts and 84.44% for the pepsin-extracts, making chicken skin a viable source of collagen. Sodium dodecylsulfate polyacrylamide gel electrophoresis showed bands at 300, 170 and 150 kDa. In the determination of arsenic, the differential pulse cathodic stripping voltammetry (DPCSV) analysis was fast optimized to determine the best combination of parameters for analysis of arsenic as arsenite. Based on the results of the optimization experiments, DPCSV was carried out with I mold, HCI, 500 mg/L Cu(11), and 4.0 mmovL thiosulfate at a potential of -500 mV, accumulation time of 150 seconds and deposition time of 100 seconds. Adsorption experiments showed that optimum arsenic removal is attained at pH 7.0 when using collagen adsorbent and at pH 6.0 when using Fe(III)-loaded adsorbent, both at one hour stirring time. The maximum removal capacities at optimum conditions were 0.785 and 0.870 mg As/g adsorbent for the collagen and Fe(111)-loaded adsorbents, respectively. Optimum adsorption capacity is obtained when using 50 mg collagen adsorbent and 20 mg Fe(111)-loaded adsorbent for 50 mL 85 pg/L arsenic solutions. Tests for reusability showed that both adsorbents can be used up to eight times without losing their removal capacity. Arsenic removal for both adsorbents was found to be highly pH-dependent.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /C355

Document Type

Thesis

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