Preparation and characterization of multilamellar liposomes containing the anti-tuberculosis agent pyrazinamide

Date

4-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

James A. Villanueva

Abstract

Liposomes are lipid bilayers that have become potential drug- delivery vehicles due to their amphiphilic structures. In this experiment, an anti-tuberculosis drug pyrazinamide was encapsulated in the liposome with 3.2 mol % DPPC:cholesterol ratio. Lipid film hydration, a widely used method, was used in the preparation of liposomes. Purification of liposomes was achieved through dialysis. The encapsulation efficiency was determined by UV-VIS Spectroscopy. The percentage of entrapment of liposomes prepared by lipid film hydration was 59.36. Characterization of liposomes was done by Stewart assay and differential scanning calorimetry. The temperature of transition of melting was Tm= 68.95C. The liposomes were viewed under a photomicroscope. The antimicrobial activity of the prepared liposome was also tested and was compared to the control, which is the pure drug.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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