"The acid and enzymatic hydrolysis of unfractionated leaf protein and fractionated chloroplastic and cytoplasmic leaf proteins from cassava (Manihot esculenta var. Vassourinha)"

Date

3-1977

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lilia S.P. Madamba

Abstract

Unfractionated leaf protein as well as fractionated chloroplastic and cytoplasmic leaf proteins were prepared from cassava (Menthot esculenta var. Vassourinha). The chloroplastic and the unfractionated leaf proteins were depigmented using acetone and alcohol. The depigmented leaf protein concentrates can compare with cytoplasmic leaf protein concentrate in terms of their color, odor, and consistency. The protein contents of unfractionated, chloroplastic, and cytoplasmic leaf protein concentrates were 51, 47.9, and 38.3%, respectively.

Acid hydrolysis of the three preparations revealed that the values of some of the essential amino acids in cytoplasmic and chloroplastic leaf protein concentrates were higher than those of unfractionated leaf protein concentrate. Partial losses of amino acids were attributed to the interaction with non-protein components of LPC during acid hydrolysis. Enzyme hydrolysis of the three LPC showed that the unfractionated and cytoplasmic leaf protein concentrates had higher values of amino acid availability than those of the chloroplastic protein concentrate.  Based on the yield of cytoplasmic leaf protein concentrate and amino acid availability.  depigmentation is preferred over the tractionation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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