Determination of the total limonoid aglycone content in the peels of calamansi (Citrofortunella microcarpa (Bunge) Wijnands) by colorimetry and densitometry

Date

4-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marlon N. Manalo

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

The total limonoid aglycones in the extracts of peels of Calamansi (Citrofortunella microcarpa) was determined by colorimetry (spectrophotometry) and densitometry (digitally-enhanced thin layer chromatography or DETLC). Limonin was extracted and purified from lime seed and used as standard for the quantification of total limonoid aglycones. Its identity was confirmed using IR, 1H-NMR and TLC analyses. For DETLC quantification, two programs were used, namely JustQuantify and TLC Analyzer. Using spectrophotometry, the total limonoid aglycones content for the dried peels and pulps of calamansi was 0.73±0.05g while using digitally-enhanced TLC, the corresponding result were 0.84±0.07 g and 0.96±0.19 g for JustQuantify and TLC Analyzer, respectively. The colorimetric and densitometric results were not significantly different at 95% confidence level. The results showed that the amount of limonoid aglycones in the peels of calamansi is significant considering the importance of limonoids. The results of this study showed that colorimetry and densitometry can both be used for the determination of total limonoid aglycones in calamansi peels and pulps as they both provide comparable results.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /V35

Notes

source: ilib only

status: in process

Document Type

Thesis

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