Development of an electrochemical biosensor for the detection of Salmonella- Specific DNA sequences
Date
4-2010
Degree
Bachelor of Science in Chemistry
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Ernesto J. Del Rosario
Committee Member
Lou Serafin M. Lozada, Conrado P. Monterola, Jr.
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Abstract
A simple and low cost electrochemical biosensor for the detection of Salmonella- specific DNA sequences was developed in this study using pencil graphite electrodes.
Polyaniline (PANI) was deposited on the electrode surface using a two-step electrochemical deposition procedure (60 μA for 3 minutes; 30 μA for 5 minutes).
Single-stranded oligonucleotide probes were covalently immobilized on the PANI- modified electrodes through the formation of phosphoramidate bonds, completing the
development of the biosensor. DNA hybridization and the evaluation of the selectivity of the biosensor were carried out. The largest differential pulse voltammetry (DPV) peak currents were observed for the ssDNA/PANI/GE electrode, three-mismatch, and non-complementary DNA hybridized biosensor, suggesting a high selectivity of the developed biosensor. A decrease in the DPV peak current occurs upon complementary DNA hybridization due to the inaccessibility of methylene blue molecules to the guanine bases. The average DPV peak currents of methylene blue were found to be linear with the logarithm of the different concentrations of complementary DNA. The limit of detection of the electrochemical biosensor to its complementary sequence is 0.3 μM.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2010 C42 C37
Recommended Citation
Carolina, Nathaniel T., "Development of an electrochemical biosensor for the detection of Salmonella- Specific DNA sequences" (2010). Undergraduate Theses. 1350.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1350
Document Type
Thesis