Micropropagation of sweetpotato (Ipomoea batatas L.) using meristems and nodal cuttings

Date

5-2019

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Lilian F. Pateña

Abstract

The study aims to develop an effective micropropagation protocol for two sweet potato cultivars to aid in the production of sweet potato that has been long declining in the Philippines due to viral infestation. The effects of stirring, fungicide and double sterilization, and media inclusion of benomyl and streptomycin in sterilization of nodal segments were determined in the study. It was found that only the inclusion of benomyl and streptomycin in the culture media reduced contamination rate of the explants. The Imelda variety resulted to lower contamination compared to the WVC Accession, suggesting that the microbial contaminants differ in the two genotypes. In plant regeneration, it was found that BAP was effective in stimulating shoot growth of meristems. For the Imelda variety, 1 mg/L was found to be the optimum concentration while 5mg/L for the WVC Accession; 5mg/L was found to be inhibitory for Imelda since lower regeneration frequency was observed. In order to induce root formation, shoots regenerated from meristems had to be transferred to the rooting medium. On the other hand, simultaneous shoot and root formation were observed from the nodal segments. The study suggests that compare plantlet regeneration of sweet potato can be obtained through the use of nodal segments and meristems.

Language

English

LC Subject

Sweet potatoes -- Micropropagation, Sweet potatoes -- Breeding

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2019 A127 M36

Document Type

Thesis

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