Association of fatty acid synthase (FASN) gene polymorphism with milk composition traits and fatty acid profile of colostrum and milk from dairy buffalo (Bubalus bubalis), cattle (Bos taurus), and goat (Capra hircus

Date

6-2023

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Orville L. Bondoc

Abstract

Fatty Acid Synthase (FASN) is an enzyme responsible for the de novo lipogenesis of longchain saturated fatty acids. This study analyzed the association of the FASN gene with major fatty acids (i.e., myristic acid C14:0, palmitic acid C16:0, stearic acid C18:0, oleic acid C18:1 n-9, and linoleic acid C18:2 n-6) and other milk composition traits in colostrum and milk of dairy buffaloes, cattle, and goats. Genotypes for the FASN gene (CC, CT, and TT) were determined using DNA extracted from hair follicles through PCR-RFLP method. The FASN genotypes were found to be polymorphic for the Holstein x Sahiwal herd (i.e., CC and CT only), but were monomorphic for Murrah and Murrah x Carabao crosses (i.e., CC only) and crossbred goats (i.e., TT only). The FASN genotypes in the local dairy herd were in Hardy Weinberg Equilibrium (HWE), with polymorphic information content (PIC) and heterozygosity (H) values of 0.2705 and 0.3225, respectively. The CT genotype was significantly associated with (P> 0.05) with higher content of C14:0 and percent protein in colostrum from Holstein x Sahiwal cows. Moreover, the test-day yield was higher in cows with CC genotype. Hence, the FASN gene may be considered as a candidate marker gene to improve C14:0 and protein content in colostrum of the local dairy herd.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2023 A127 M46

Document Type

Thesis

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