Preliminary studies on the production of laccase by Trametes versicolor Biotech 3180 in different media, incubation period and initial pH

Date

10-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ida F. Dalmacio

Co-adviser

Fides Z. Tambalo

Restrictions

Restricted: Not available to the general public and for consultation with the author/thesis adviser. Access is available only to those bound by the confidentiality agreement.

Abstract

Trametes versicolor is a white-rot fungi which is able to produce laccase and the BIOTECH strain of this organism was used in this study. The main objective of the study was to determine the production of laccase by the test organism in four different media: corn cobs broth, rice bran broth, cassava starch broth and potato dextrose broth at various incubation periods and in varying initial pHs. The volumetric activity (U/mL) of the laccase produced was determined using syringaldazine assay. F-test and Duncan?s Multiple Range Test were used in the statistical analysis to determine the significant differences among treatments and to determine the conditions for production of laccase with the highest volumetric activity. Based on the results, of the media tested, corn cobs gave the highest value. A 7- day incubation period and an initial pH of 5.5 of the medium also showed significantly higher enzyme yield and activity.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 B4 /C33

Document Type

Thesis

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