Analgesic and toxic activities of the methanolic extract of the purple fluid of the sea hare Dolabella auricularia (Lightfoot, 1786)

Date

10-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Zoology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Maria Dalisay G. Maligalig

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

Methanolic extract of the purple fluid secreted by the Palawan Dolabella auricularia was evaluated for its analgesic and toxic properties. For the analgesic assay, the abdominal writhing method was conducted on 72 mice randomly distributed into three experimental set-ups. Each set up has: distilled water (negative control), morphine (positive control), 25% and 50% (v/v) extract groups. Behavioral responses and mortality were also monitored. Toxicity was assessed using the brine shrimp lethality assay with six extract concentrations and K2Cr2O7 as positive control. Results from the three trials show that the mean percent inhibition of abdominal writhing were significantly different among treatment groups (p<0.05) with higher percent inhibition in the 50% (97.5%) and 25% (80.4%) extracts compared to the morphine (67.1%) groups. Reduced grooming and locomotory activities as well as presence of watery excretions were observed in all extract-treated mice. Mortality rate was highest in the 50% extract group. LC50 values derived from the brine shrimp lethality assay were established at extract concentrations of 807.7 (6 h), 449.5 (12 h), and 300.1 μL/mL (24 h). Data suggest that the purple fluid of D. auricularia has both analgesic and toxic properties. However, further evaluation of the components and properties of the secretions of the sea hare is still necessary.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 B4 /E44

Document Type

Thesis

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