Isolation and identification of alkaliphilic bacteria from Manleluag hyperalkaline spring in Pangasinan and screening for cellulose and protease activity

Date

10-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Nacita B. Lantican

Committee Member

Wilma T. Cruz Nina M. Cadiz

Abstract

The identity and the ability of 14 alkaliphilic isolates obtained from water samples from Manleluag Hyperalkaline Spring to produce extracellular proteases and cellulases were studied. Through observations on morphology and cultural characteristics on Horikoshi-I medium as well as analysis of 16S rRNA gene sequence, the alkaliphilic isolates were identified to belong to different strains under four genera namely, Bacillus, Aeromicrobium, Exiguobacterium, and Lysinibacillus?all of which are known alkali-tolerant to alkaliphiles. Extracellular protease production determined using the Skim Milk Assay and Gelatin Assay showed that 13 out of 14 isolates were able to produce extracellular proteases. Through calculation of Cz values and DMRT analysis, the best extracellular protease producer were determined to be isolate W12 (E. mexicanum) with an overall mean Cz value of 0.2406. Analysis revealed that the said isolate exhibited significantly higher extracellular protease production as compared to B. subtilis (Cz 0.4024). Only isolate W8 which was identified as Aeromicrobium alkaliterrae KSL-107 (Cz 1.0000), was not able to produce extracellular proteases thus, its inability to degrade the casein and gelatin in the assay media. Extracellular cellulase production determined using a Basic Mineral Medium supplemented with 0.3% carboxymethyl cellulose showed that only isolate W14 which was identified to be L. sphaericus TB-22 (Cz 0.1211) was able to produce zones of clearing on the plates indicative of its ability to produce a significant amount of extracellular cellulase.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 B4 /M67

Document Type

Thesis

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