DNA barcoding and genetic diversity using mtCOI gene in mango gall midge Procontarinia frugivora Gagne and P. pustulata (Diptera: Cecidomyiidae) from San Ildefonso, Bulacan, Philippines
Date
4-2014
Degree
Bachelor of Science in Biology
Major Course
Major in Cell Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Melvin S. Mendioro
Co-adviser
Celia dR. Medina
Restrictions
Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.
Abstract
DNA Barcoding and genetic diversity were analyzed using mitochondrial COI gene in mango gall midge Procontarinia frugivora and P. pustulata from San Ildefonso, Bulacan. The universal insect primer set, LCO 1490 and HCO 2198, were used for the amplification of mtCOI gene. Pairwise distances suggested little or no transition/transversion mutation within P. frugivora and P. pustulata. Results of Tajima's Relative Rate Test signified an equal evolutionary rate between the two species. Sequence alignment indicated a percent homology of 82.8% between the two species. Construction of maximum likelihood (ML) tree showed separate clustering of the two species. Based on these results, DNA barcoding using mtCOI gene could delineate P. frugivora from P. pustulata.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2014 B4 /R66
Recommended Citation
Ronquillo, Sarah Joy Q., "DNA barcoding and genetic diversity using mtCOI gene in mango gall midge Procontarinia frugivora Gagne and P. pustulata (Diptera: Cecidomyiidae) from San Ildefonso, Bulacan, Philippines" (2014). Undergraduate Theses. 1401.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1401
Document Type
Thesis