DNA barcoding and genetic diversity using mtCOI gene in mango gall midge Procontarinia frugivora Gagne and P. pustulata (Diptera: Cecidomyiidae) from San Ildefonso, Bulacan, Philippines

Date

4-2014

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Melvin S. Mendioro

Co-adviser

Celia dR. Medina

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

DNA Barcoding and genetic diversity were analyzed using mitochondrial COI gene in mango gall midge Procontarinia frugivora and P. pustulata from San Ildefonso, Bulacan. The universal insect primer set, LCO 1490 and HCO 2198, were used for the amplification of mtCOI gene. Pairwise distances suggested little or no transition/transversion mutation within P. frugivora and P. pustulata. Results of Tajima's Relative Rate Test signified an equal evolutionary rate between the two species. Sequence alignment indicated a percent homology of 82.8% between the two species. Construction of maximum likelihood (ML) tree showed separate clustering of the two species. Based on these results, DNA barcoding using mtCOI gene could delineate P. frugivora from P. pustulata.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 B4 /R66

Document Type

Thesis

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