Virus detection in tissue cultured abaca (Musa textilis Nee) by serological and molecular methods

Date

4-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Filomena C. Sta. Cruz

Abstract

Diseases caused by Banana bunchy top virus (BBTV), Banana bract mosaic virus (BBrMV) and Sugarcane mosaic virus (SCMV) are serious problems affecting abaca production. This study aimed to establish a reliable virus indexing scheme using serological and molecular virus detection methods in order to ensure the production of virus-free abaca planting materials through tissue culture. The enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) were used to detect the presence of the virus in in vitro and ex vitro abaca cultures. BBTV, BBrMV and SCMV were detectable by ELISA with infection of more than 50% and even higher up to 100%. Mixed infection occurred with two or three viruses together. The presence of BBTV was also confirmed by PCR detection, but template DNA dilution seemed to affect the efficiency of virus detection. The in vitro and ex vitro cultures had inconsistent reaction in the test for BBTV, BBrMV and SCMV, although the samples analyzed were obtained from the same tissue culture line. Among the in vitro cultures, samples of the first, second or third subculture had also inconsistent reaction. However, the number of BBrMV positive samples appeared to be reduced with subsequent subculture. This observation was not apparent for BBTV and SCMV. Some of the in vitro cultures showed symptoms of vein chlorosis, vein necrosis and leaf deformation. Some symptomatic samples were found to be positive thus, the symptoms expressed by the plant is due to virus infection. However, the symptom expressed may not be attributed to virus infection, since not all the symptomatic plantlets were positive to the virus. These symptoms can be morpho-physiological disorders due to the effect of some unfavorable in vitro culture conditions. On the other hand, some in vitro plantlets and the ex vitro cultures were virus positive but had not expressed any virus-like symptom.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A3 /L36

Document Type

Thesis

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