Genetic relationship between Philippine native and ancient polynesian chickens (Gallus gallus domesticus L.) inferred through DNA analysis of mitochondrial D- loop region

Date

12-2014

Degree

Bachelor of Science in Agricultural Biotechnology

Major Course

Major in Animal Science

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Medino Gedeun N. Yebron, Jr.

Abstract

This study was carried out to identify the maternal lineage of eight genetic groups of Philippine native chicken (Labuyo, Darag Boholano, Paraoakan, Bolinao, Banaba, Camarines and Joloano) and examine the claim of a recent study on the migration of the Polynesians that stated that the Philippines is the "potential homeland" of the Polynesian chicken. The mitochondrial D-loop region of 27 chickens from eight genetic groups of chicken was sequenced. The highly variable 201bp segment of the D-loop region was used for analysis and 16 haplotypes were identified from 24 polymorphic sites. Phylogenetic analysis showed that the Philippine native chickens can be grouped into five clades (A,B,D,E and H) of the previously identified 13 clades. Sixteen (16) samples grouped in Clade D, eight grouped in Clade E, two in Clade B and one in Clade A and Clade H. The results indicated multiple maternal origin of the Philippine native chickens. The study tried to verify the claim of the study of Thomson et al. (2014) that the Philippines is the possible homeland of the Polynesian chickens. Samples under haplogroup D were examined if they possess all four diagnostic SNPs, that define the true maternal lineage of the Polynesian chicken. No sample was found to possess all diagnostic SNPs. However, some samples of Paraoakan, Joloano, Boholano and Labuyo were found to have haplotypes that differ only by one mutation to the most common ancient Polynesian chicken haplotype, making them possible candidates as the ancestors of the Polynesian chicken.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 /P47

Document Type

Thesis

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