Comparative duodenal alpha-amylase activity of broilers fed diets with varying levels of essential oils and benzoic acid mixture

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

Major Course

Major in Animal Science

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Amado A. Angeles

Abstract

To compare the duodenal α-amylase activity of broilers fed varying levels of essential oils and benzoic acid (EO/BA) and an antibiotic growth promoter (Avilamycin), a feeding trial involving 20 day-old broilers were divided into 4 groups with 5 replicates per group and were given basal diet (negative control) as treatment 1 (T1), T1 +10 ppm of Avilamycin (positive control), T1 + 150 ppm and T1 + 300 ppm EO/BA of feed following CRD. Thirty five day growth performance parameters including feed consumption (FC), body weight gain (BWG), feed conversion ratio (FCR), and livability were determined. After slaughter, duodenal contents were subjected to α-amylase activity using DNSA assay and duodenal protein profiling by SDS-PAGE. Results showed no significant differences (P>0.05) in all growth parameters and duodenal α-amylase activity in broilers. Electrophoretogram showed that the α-amylase protein profile of different treatments have similar molecular weight of 55.98 kDa, and the quantity of α-amylase concentration was related to the intensity of bands in SDS-PAGE. Under the condition from which this study was conducted, results indicate that feeding broilers with varying levels of EO/BA mixture and Avilamycin do not have significant effects on duodenal α-amylase activity and growth performance of the broilers.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 S26

Document Type

Thesis

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