Research internship on the comparative duodenal bacterial diversity and growth performance of broilers fed diets supplemented with antibiotic growth promoters and Bacillus subtilis

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Amado A. Angeles

Abstract

The research internship aimed to familiarize the intern with various molecular biotechnology techniques such as DNA extraction, Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) while at the same time developing the intern's professionalism and work ethics which are necessary in that field of study. The goal of the study was to determine the effects of varying diets: basal feed with antibiotic growth promoters (AGP) and probiotic (Bacillus subtilis), basal feed with AGP only and basal feed with Bacillus subtilis only on the bacterial diversity in the duodenum and growth performance of broilers. Two feeding trials were done during the study which made use of 630 and 21 day-old chicks. The bacterial diversity was assessed through the subjection of the amplified V3 to V5 regions of the 16S bacterial rDNA to DGGE. Analysis of the results of the growth performance of broilers through ANOVA in CRD showed that both AGP and Bacillus subtilis had no effect on the growth performance of broilers. Results obtained from DGGE showed that there are 9 possible different species of bacteria present in the duodenum. Through the research internship, the intern was able to gain knowledge and acquire skills on various biotechnology techniques and principles; hence developing the intern's insights extensively on the research industry.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2015 A127 /A24

Notes

Major in Animal Biotechnology

Document Type

Thesis

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