Screening and characterization of ethanol-tolerant yeast and amylase-producing yeast species in fermenting taro [Colocasia esculenta (L.) Schott] with rice in Leyte, Philippines
Date
7-2015
Degree
Bachelor of Science in Agricultural Biotechnology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Reina Esther Servan Caro
Co-adviser
Katherine Ann C. Israel
Committee Member
Susana M. Mercado
Abstract
The study aimed to screen for ethanol-tolerant yeasts and amylase-producing yeasts for the formulation of microbial starter for alcoholic and non-alcoholic beverages. A total of 37 pure yeast isolates from fermenting taro-rice wine in Leyte, Philippines and two isolates, identified as Saccharomycopsis sp. by the Institute of Food Science and Technology, UPLB, were characterized and grouped based on their morphology. Moreover, microsatellite (GTG)5 fingerprinting was used to further group them based on their genetic patterns. Twenty-one different genetic patterns of yeast isolates from fermenting taro-rice and two isolates with known genera were observed. One representative species from each of the distinct groups was subjected to ethanol tolerance test and amylase production test. Top three isolates with the highest growth at 10% and 12% ethanol concentration were selected. Moreover, top four isolates found to produce amylase were selected and compared with the two Saccharomycopsis sp. known as amylase producers. The selected yeast isolates from ethanol-tolerance test were found to be different from the yeast isolates screened to have amylase production; however, the exact identities of these isolates cannot be determined unless further evaluation and identification like DNA sequencing and phylogenetic analysis are done.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2015 A127 /C37
Recommended Citation
Caro, Reina Esther Servan, "Screening and characterization of ethanol-tolerant yeast and amylase-producing yeast species in fermenting taro [Colocasia esculenta (L.) Schott] with rice in Leyte, Philippines" (2015). Undergraduate Theses. 1612.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1612
Document Type
Thesis
Notes
Major in Food Biotechnology