Genetic characterization of Philippine native chickens (Gallus gallus domesticus L.) through SSR analysis

Date

12-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Medino Gedeun N. Yebron Jr.

Abstract

The study was conducted to characterize the genetic diversity within population of 8 genetic groups of Philippine native chickens (Paraoakan, Darag, Camarines, Bolinao, Boholano, Banaba, Joloano and Labuyo) together with one commercial breed (Layer) through SSR analysis. DNA was extracted from chicken blood samples of FTA cards and seven SSR loci were used to amplify through polymerase chain reaction. PCR products were visualized through agarose and polyacrylamide gel electrophoresis. Based on the scorable bands, 5 out of the 7 SSR markers were used for analysis. The mean number of observed allele, mean observed heterozygosity and Fis ranges from 4.2 to 6; 0.6232 to 0.7950; 0.01564 to -0.20304, respectively.Paraoakan (Ho and Fis) and Boholano (MNA) revealed to have high genetic diversity. All the 8 genetic groups of Philippine native chicken were distinct from each other based on the Fst value. The cluster analysis also revealed that Labuyo was the descendant of most Philippine native chicken except for Paraoakan and Joloano. This indicates that Paraoakan and Joloano could have a different ancestor, not the Labuyo or the Philippine Red Jungle Fowl. Furthermore, the overall diversity observed in the population studied may also be attributed to the small sample size and number of microsatellite loci analyzed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 /F33

Notes

Major in Animal Biotechnology

Document Type

Thesis

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