Single-marker analysis for induced resistance in tomato (Solanum lycopersicum L.) against the tomato leaf curl Philippine virus Los Baños isolate

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Hayde F. Galvez

Abstract

A total of eleven (11) mutant tomato plants from three (3) M2:3 families derived from previous rounds of selection in the Tomato Genomics Project were used in the study. Among the mutant lines, two (2) M3 plants from 947-23 were both rated as Resistant and Highly Resistant. In the polymorphism screening, 12 SSR markers based on Sol Genomics Network (SGN) database were used to screen for DNA profile variation within mutant lines, and in comparison with H7996 and wild-type S. pimpinellifolium control genotypes. With no observed significant SSR polymorphism within the mutant lines, the test plants were subjected to CG gene-specific marker and agarose-based Targeting Induced Local Lesions in the Genome (TILLING) analyses. The mutant plants were also screened for mutation points at Ty (Ty-1, Ty-2, and Ty-3) gene regions. The resistance of the 947 mutant line was further evaluated with larger-progeny population size including sister lines. Based on Single-Factor Analysis of Variance (ANOVA) at 0.05 statistical confidence, marker association was found significant within the 947-23 mutant lines but not validated in the larger-scale experimental setup. For future studies, larger sample size especially progeny of the exact mutant family of line 947, and more advanced statistical tools are highly recommended.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /H33

Notes

major: Crop Biotechnology

Document Type

Thesis

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