Screening of starch degrading fungi and evaluation of their activities in cassava (Manihot esculenta) and taro [Colocasia esculenta (L.) Schott] starch

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Katherine Ann C. Israel

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

Seventeen (17) mold cultures were isolated from fermenting taro-rice combination using starter cultures specifically ragi tape and bubod. These were screened and evaluated for their hydrolyzing activities and were grouped according to the type of starch they hydrolyze more in terms of their α-Amylase and glucoamylase activities. Out of 17 isolates, ten isolates exhibited high α-Amylase activity in taro and seven isolates exhibited high α-Amylase activity in cassava. For glucoamylase activities, eleven isolates showed high activities in taro and six isolates showed high activities in cassava. Isolates RtpFm-2 and RtpFm-5 can be used in taro based products and isolates BKFm-1 and RttFm-1 can be used in cassava based products. Further analysis showed that reducing sugar concentration was produced by glucoamylase. Also, it can be pointed out that glucoamylase is a primary metabolite of RtpFm-2 and RtpFm-5 for both concentrations of taro and a primary metabolite of RttFm-1 for both concentrations of cassava starch. Furthermore, it can be pointed out that sugar concentration favors biomass formation. From this, it can be concluded that reducing sugar and taken up by the microorganism to form biomass.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

993.5 2015 A127 /K34

Notes

major: Food Biotechnology

Document Type

Thesis

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