Research internship on thrifty genotype breeds involving leptin gene polymorphism of commercial pigs on Luz Farms, Inc.

Date

7-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Renato SA. Vega

Abstract

The research internship was conducted at the Animal Biotechnology Laboratory at the Animal and Dairy Sciences Cluster, College of Agriculture, University of the Philippines Los Baños, College Laguna from May 2013 to December 2014. The objective of the project was to determine if different economically important traits were associated with backfat thickness and leptin gene polymorphism. The student objective in the research internship is to gain confidence in collecting hair follicle and farm data and conduct DNA extraction, agarose gel electrophoresis, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), statistical analysis and data interpretation. The student took part in various farm and laboratory activities. These include sample collection and preparation, DNA extraction, PCR-RFLP, and gel documentation. Initial results showed that there were no associations between leptin genotypes and economically important traits and backfat thickness, this is because not all genotypes were represented. The absence of the CC genotype may be due to the effect of long ? term selection pressure resulting to elimination of CC genotype (Thrifty Genotype). Power outages at the laboratory and the absence of the student due to classes were the problems experienced during the internship. Increasing the sample size and random sampling may help increase the chance of CC genotype to occur.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2015 A127 /M38

Notes

Major: Animal Bitechnology

Document Type

Thesis

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