Pretreatment and saccharification of Talahib grass (Saccharum spontaneum) prior to ethanol fermentation by Saccharomyces cerevisiae and Pichia stipitis

Date

10-2010

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ernesto J. Del Rosario

Co-adviser

Veronica P. Migo

Committee Member

Joy Elaine P. Kaligayahan,

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Abstract

Talahib grass (Saccharum spontaneum) was dried, ground and subjected to compositional analysis. The cellulose content was 42.7+0.03% while the lignin content was 35.1+0.60% and 2.20+0.60% for acid-soluble and acid-insoluble, respectively. The glucose content of the sample was determined to be 30.8+17.2% using HPLC. Talahib was then pretreated with 0.6%(v/v) sulfuric acid for 30 minutes in an autoclave at 121°C (15psi). The pretreated substrate was oven-dried and saccharified with either 0.4%(v/v) sulfuric acid at 121°C for 30 minutes or cellulolytic enzymes (Novozymes Biomass Kit or Genencor Accelerase 1500) at 50°C for 72 hours with constant shaking. The total sugar concentration was 3.22+1.38% for solid-to-liquid ratio of 1:5 in acid hydrolysis. Total sugar concentrations obtained using Genencor Accelerase 1500 and Novozymes Biomass Kit are 2.09+0.05% and 2.26+0.05%, respectively. Total reducing sugar analysis, on the other hand, resulted to 2.34+0.04%, 2.08+0.11% and 2.22+0.06% for acid hydrolysis, Genencor Accelerase 1500 and Novozymes Biomass Kit methods, respectively. The hydrolysates that gave the highest amount of sugar after saccharification were subjected into sequential ethanol fermentation using Saccharomyces cerevisiae BIOTECH 2030 and Pichia stipitis (NRRL y-7124). The maximum ethanol yields were 0.71% (v/v), 0.58%(v/v) and 0.66%(v/v) for dilute acid, Genencor Accelerase 1500 and Novozymes Biomass Kit hydrolysates, respectively. The results have shown that talahib is an efficient substrate for ethanol fermentation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2010 C42 C43

Document Type

Thesis

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