Isolation and partial purification of the crude protein from cashew nut (Anacardium occidentale), peanut (Arachis hypogaea L. ), and pili nut (Canarium ovatum Engl.) exhibiting angiotensin converting enzyme inhibitory activity
Date
10-2010
Degree
Bachelor of Science in Chemistry
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Mary Ann O. Torio
Co-adviser
Roberta N. Garcia
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Abstract
The crude proteins of peanut, cashew nut and pili nut were isolated using different extraction buffers. For peanut and cashew nut, protein extraction buffer containing 0.02 M Tris-HCl with pH 8.11 and 8.2 were used. For pili nut, 0.5 M NaCl was used. The protein content was determined using Bradford method. The protein content of peanut, cashew nut and pili nut were found to be 22.77 mg/mL, 17.66 mg/mL and 12.64 mg/mL, respectively. Partial purification of the crude proteins of three samples was done by ammonium sulfate fractionation and dialysis. In vitro digestibility of the crude proteins by proteases was carried out using trypsin, chymotrypsin, and pepsin. Enzyme hydrolysates of pepsin, trypsin and chymotrypsin after 24 hours digestion were subjected to angiotensin I-converting enzyme (ACE) inhibitory activity assay. Pepsin hydrolysate from peanut and cashew nut resulted to 2.93 x 10-3 and 2.06 x 10-3 inhibitory activity units, respectively. Pepsin, trypsin, and chymotrypsin hydrolysates of pili nut resulted to 3.13 x 10-3, 3.30 x 10-3, and 3.26 x 10-3 inhibitory activity units, respectively. Thus, peanut, cashew nut and pili nut contain bioactive peptides with anti-ACE (angiotensin I- converting enzyme) activity.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Recommended Citation
Ite, Michelle O., "Isolation and partial purification of the crude protein from cashew nut (Anacardium occidentale), peanut (Arachis hypogaea L. ), and pili nut (Canarium ovatum Engl.) exhibiting angiotensin converting enzyme inhibitory activity" (2010). Undergraduate Theses. 1835.
https://www.ukdr.uplb.edu.ph/etd-undergrad/1835
Document Type
Thesis