Morphological, cytogenetic, and molecular characterizations of Oryza granulata Nees et Arn. ex Watt and its hybrids with Oryza sativa L.

Date

4-2014

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Co-adviser

Kshirod K. Jena

Abstract

Morphological, cytogenetic, and molecular analyses of Oryza sativa L. tetraploid IR64 (4n), O. granulata Acc. 102118 and their progenies, WHC 4324 and WHC 4319, were conducted to determine if the F1s were true hybrids. The overall morphologies of the hybrids resembled more the IR64 parent with some traits similar with the O. granulata parent. The cytogenetic analysis revealed that the chromosome number of the F1s were 2n=36 since most of the cells at diakinesis had chromosome configuration of 12 II + 12 I. Molecular analysis detected the presence of both genomes in the hybrids through the polymorphism observed in four out of 766 SSR markers used, namely RM 165 in chromosome 1, RM 159 in chromosome 5, RM 6701 in chromosome 6, and RM 172 in chromosome 7. From all the characterizations made, it is concluded that WHC 4324 and WHC 4319 are both true hybrids of the cross IR64 (4n) x O. granulata.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 B4 /M36

Notes

Major in Cell and Molecular Biology

Document Type

Thesis

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