Determination of total polyphenol and flavonoid contents and antioxidant property of pollen gathered by Tetragonula biroi Friese from select apiaries in Batangas, Laguna and Sorsogon

Date

10-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jose Rene L. Micor

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The total polyphenol and flavonoid content and antioxidant property of bee pollen loadsfrom six towns in Southern Luzon were determined. Pollen loads collected from each town were further sorted according to color resulting to 14 samples. The total polyphenol and flavonoid content of the ethanolic sample extracts were determined by Folin-Ciocalteu and Dowd methods respectively, while the antioxidant property was determined in vitro through diphenylpicrylhydrazyl (DPPH) radical scavenging assay, hydrogen peroxide (H2O2) savenging assay and ferric reducing antioxidant power (FRAP) assay. Higher polyphenol content and flavonoid content and antioxidant activity were measured in multifloral and darker bee pollen. Highest polyphenol content was measured in orange bee pollen of Santa Cruz (Combreteceaetype), with 19.72 ± 1.96 mg GAE/g extract while highest flavonoid content was measured in brown bee pollen of Calatagan (multifloral) with 5.62 ± 0.51 mg QE/g extract. Highest antioxidant activity was measured in brown bee pollen of Calatagan (multifloral) both for DPPH assay with EC50of 3.72 ± 0.30 mg/mL and FRAP assay with 0.60 ± 0.06mmol Fe2+/g extract,and orange bee pollen of Alaminos (C. Nucifera) for H2O2 assay with 62.81 ± 4.19 %. Lowest polyphenol content and lowest flavonoid content weremeasured in orange bee pollen of Calatagan (Apocynaceaetype)with 10.24 ± 0.49 mg GAE/g extractand 1.55 ± 0.13 mg QE/g extract, respectively. Lowest antioxidant activity was alsomeasured in orange bee pollen of Calatagan for DPPH assay with EC50of 12.75 ± 0.92 mg/mL and FRAP assay with 0.23 ± 0.03mmol Fe2+/g extract and yellow bee pollen of Santa Cruz (Compositaetype 1) for H2O2 assay with 25.48 ± 2.93 %.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /M46

Document Type

Thesis

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