In-vivo screening of the antagonistic activity of lactic acid bacteria on Paenibacillus larvae subsp. larvae isolated from European honeybees (Apis mallifera L. )

Date

2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Jaymee R. Encabo

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Abstract

A total of five Gram positive, endospore-forming, catalase negative rod-shaped bacteria were isolated from diseased larvae of European honeybees, Apis mellifera. Physiological characterization revealed that two of the five isolated organisms were both Paenibacillus larvae subsp. larvae. Pathogenicity testing using honeybee brood showed that the isolated Paenibacillus was capable of infecting both A. mellifera and the native bee, A. cerana. Moreover, the identity of the isolate was confirmed because of the symptoms of American foulbrood exhibited by the inoculated brood. Three strains of Lactobacillus plantarum, reported to exhibit inhibitory effect against Paenibacillus larvae in vitro were screened for their antagonistic activity against the pathogenic isolate. A total of 800 A. cerana and 840 A. mellifera larvae were subjected to treatments involving different combinations of lactic acid bacteria and spores of the bee pathogen. Results showed that all of the lactic acid bacteria were capable of significantly inhibiting the growth of Paenibacillus larvae in vivo.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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