Bacterial community in feces of domesticated pigs (Sus scrofa domestica Linn.) fed with conventional and probiotic-supplemented diet as revealed by denaturing gradient gel electrophoresis analysis of 16s rDNA

Date

2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Nacita B. Lantican

Request Access

To request access of this material, please email the administrator at uscs-mainlib.uplb@up.edu.ph

Abstract

Denaturing gradient gel electrophoresis (DGGE) was used to assess the difference in the profiles of the bacterial community in fecal matter collected from the distal colon of six domesticated pigs fed with corn-based diet with and without PSB 1X probiotic supplement after 105 days. Community DNA was extracted from the fecal samples using MOBIO UltraClean® Fecal DNA Isolation Kit. Near full-length 16S ribosomal DNA was amplified through PCR using bacteria-specific forward primer 8F and universal reverse primer 1492R. The V2-V3 hypervariable region of 16S ribosomal DNA was amplified

through nested PCR using bacteria-specific forward primer HDA-1-GC and bacteria- specific reverse primer HDA-2.

Basic Local Alignment Search Tool (BLAST) result of the 11 excised DGGE bands rendered mostly identities of uncultured bacteria; though three genera of known fecal bacteria were detected namely, Clostridium, Ruminococcus and Eubacterium. One-way analysis of variance (ANOVA) showed that the adopted regimen of probiotic supplementation did not cause any statistically significant changes in the species richness (S), biodiversity (H), and dominance (C) in the bacterial community in feces. Based on analysis of DGGE patterns, probiotic supplementation does not seem to have any significant effect on diversity in the fecal bacterial community; however, the change in its composition could not yet be determined since majority of the sequenced fragments were identified to have come from non-cultivable bacteria.

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

This document is currently not available here.

Share

COinS