Estimation of genetic variation in populations of freshwater prawn Macrobrachium lanceifrons (Dana) from Los Baños and San Pablo, Laguna and Nasugbu, Batangas through isozyme analysis using starch gel electrophoresis

Date

2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ma. Carmina C. Manuel

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Abstract

Genetic variation in freshwater prawn Macrobrachium lanceifrons from Los Baños and San Pablo, Laguna and Nasugbu, Batangas was assessed through isozyme polymorphism. Seven presumptive loci were detected in the three populations based on

four enzyme systems. Two isoloci detected were for acid phosphatase (ACP-1 and ACP- 2), two for alkaline phosphate (ALP-1 and ALP-2), two for esterase (EST-1 and EST- 2) and one for malic enzyme (ME-1). ACP-1, ALP-1, and EST-1 were controlled

by two alleles S and F, while ACP-2, ALP-2, EST-2 and ME-1 were controlled by a single allele S. ACP-1 was found to be polymorphic while ACP-2 was monomorphic in all populations. ALP-1 was polymorphic except in the San Pablo population, while

ALP-2 was monomorphic except in the Los Baños samples where it was absent. EST- 1 was polymorphic while EST-2 was monomorphic in all populations. ME-1 was

monomorphic in all populations. Genetic variation within the population was low as seen from the proportion of polymorphic loci (P= 0.286-0.500), average heterozygosity (H= 0.122-0.209) and average number of alleles (A = 1.286 – 1.500). The eye and muscle tissues of the prawn samples showed a slight difference in isozyme expression but the values of the parameters measured did not differ much. The low genetic variability within each population could be possibly explained by inbreeding. Genetic variation between populations was also low as shown by values for genetic distance (D= 0.036-0.199) and a high genetic relatedness was indicated by estimates for genetic identity (IN= 0.820-0.965) and genotypic similarity (IH= 0.872-0.952). The data gathered suggests low genetic variation within and between populations of M. lanceifrons considered in the study. It is also possible that the genetic variation may not have been fully shown based on the enzyme systems used.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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