Morpho-agronomic characterization and microsatellite-based genetic diversity analysis of different thermosensitive genetic male sterile (TGMS) lines of rice (Oryza sativa L.)

Date

2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ma. Genaleen Q. Diaz

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Abstract

TGMS lines obtained from the PhilRice-UPLB hybrid rice breeding program were used to characterize 12 morphological traits and seven agronomic characters and to evaluate the genetic relationship and diversity based on microsatellite polymorphism. Minimal diversity was observed among the 21 lines evaluated as indicated by the high degree of similarity in 11 out of 12 morphological traits. However, the agronomic traits evaluated were variable among the lines. Thirty-nine (39) microsatellite markers were used to examine the genetic diversity of 22 TGMS lines. Out of the 112 alleles detected, 31 (27.68%) were monomorphic. A 100% polymorphism was calculated in 16 loci (41.03%) while polymorphism ranged from 0% to 75% in the other loci. A total of 17 loci (43.59%) had no polymorphism. Based on the number of alleles detected and PIC, only one marker (RM 169) can be considered as a highly informative marker since it satisfies both criteria. At a 64% level of similarity, the dendrogram showed three major clusters with additional sub-clusters within each group. The similarity coefficients between any two TGMS lines ranged from 59% to 79% in cluster I, from 61% to 71% in cluster II, and from 61% to 100% in cluster III. The bootstrap values for the three major clusters were substantially lower than the 95% confidence rule of bootstrapping. There were, however, smaller subgroups in cluster III with very high bootstrap support. Two lines, TG17 and TG20, were separated from the other lines with highest bootstrap support of 99.3%. The sub-group containing six TGMS lines (TG7, TG12, TG13, TG15, TG17, TG18 and TG20) also had the high bootstrap value (96%). The morpho-agronomic and genetic characterization showed high degree of similarity among the TGMS lines evaluated.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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